Carnegie Mellon University                              

Link to CMU/Biological Physics/Nagle,Tristram-Nagle Website here

Link to Lab’s Software here

My research interests are in lipid membrane structure, thermodynamics and fluctuations. Our Biophysics laboratory explores the structure of biomimetic membranes containing phospholipids, peptides and cholesterol mixtures using oriented samples on substrates and unoriented multilamellar or large unilamellar vesicles.  We use X-ray diffraction with a Rigaku rotating anode and CCD detector here at CMU, and synchrotron radiation at the Cornell High Energy Synchrotron Source to obtain diffuse scattering data that yields not only structure but also elastic parameters. Our recent experiments also use neutron scattering.  Another technique is differential scanning calorimetry to measure specific heat changes during phase transitions of aqueous dispersions of phospholipids in the form of MLVs. The fourth technique which yields structural information about the lipid volume as a function of temperature is neutral flotation using D2O-H2O mixtures and our DMA5000M vibrating tube densimeter.

 Recent Publications:

1.      Nagle, J.F., M.S. Jablin, Tristram-Nagle, S. 2016. Sugar Does Not Affect the Bending and Tilt Moduli of Simple Lipid Bilayers.  Chem. Phys. Lipids 196:76-80. (PDF) SM (PDF)

2.      Nagle, J.F., Akabori,  K., Treece, W., Tristram-Nagle, S. 2016. Determination of Mosaicity in Oriented Stacks of Lipid Bilayers.  Soft Matter 12:1884-1891. (PDF) SM (PDF)

3.      Tristram-Nagle, S. 2015.  Use of X-Ray and Neutron Scattering Methods with Volume Measurements to Determine Lipid Bilayer Structure and Number of Water Molecules/Lipid. In Membrane Hydration, The Role of Water in the Structure and Function of Biological Membranes, Subcellular Biochemistry Vol. 71. Ed. E. Anibal Disalvo, Springer International Publishing AG, Switzerland. (PDF)

4.      Neale, C., Huang, K., Garcia, A.E.,  Tristram-Nagle, S. 2015.  Penetration of HIV-1 Tat47-57 into PC/PE bilayers assessed by MD simulation and X-ray scattering.  Membranes 5:473-494. (PDF) Supplementary Materials (PDF)  MovieSM1 (mp4)  MovieSM2 (mp4)

5.      Reese, C.W., Strango, Z.I., Dell, Z.R., Tristram-Nagle, S. , Harper, P.E. 2015.  Structural insights into the cubic-hexagonal phase transition kinetics of monolein modulated by sucrose solutions.  Physical Chemistry Chemical Physics 17:9194-9204. (PDF)

6.      Ma, Y., Ghosh, S.K., Bera, S., Jiang, Z., Tristram-Nagle, S., Lurio, L.B., Sinha, S.K. 2015.  Accurate calibration and control of relative humidity close to 100% by X-raying a DOPC multilayer.  Physical Chemistry Chemical Physics 17:3570-3576. (PDF)

7.      Nagle, J.F., Jablin, M.S., Tristram-Nagle, S. ,  Akabori, K. 2015.  What are the true values of the bending modulus of simple lipid bilayers?  Chemistry and Physics of Lipids 185:3-10. (PDF)

8.      Akabori, K., Huang, K., Treece, B.W., Jablin, M.S., Maranville, B., Woll, A., Nagle, J.F., Garcia, A.E., Tristram-Nagle, S. 2014.  HIV-1 Tat membrane interactions probed using X-ray and neutron scattering, CD spectroscopy and MD simulations.  Biochimica et Biophysica Acta 1838:3078-3087. (PDF) Supporting Information (PDF)

 9.     Boscia, A., Treece, B.W., Mohammadyani, D., Klein-Seetharaman, J., Braun, A.R., Wassenaar, T.A., Klösgen, B., Tristram-Nagle, S.  2014.  X-ray structure, thermodynamics, elastic properties and MD simulations of cardiolipin/ dimyristoylphosphatidylcholine mixed membranes.  Chemistry and Physics of Lipids 178:1-10 (PDF).

10.    Boscia, A., Akabori, K., Benamram, Z., Michel, J.A., Jablin, M.S., Steckbeck, J.D., Montelaro, R.C., Nagle, J.F. and Tristram-Nagle, S. 2013.  Membrane Structure Correlates to Function of LLP2 on the Cytoplasmic Tail of HIV-1 GP41 Protein.  Biophysical Journal 105:657-555. (PDF)  Supporting Information (PDF)

 11.   Pogozheva, I.D., Tristram-Nagle, S., Mosberg, H.I. and Lomize, A.L. 2012. Structural Adaptations of Proteins to Different Biological Membranes.  Biochimica et Biophysica Acta 1828:2592-2608. (PDF)  Supporting Information (PDF)

 12.   Pan, J., Heberle, F.A., Tristram-Nagle, S., Szymanski, M., Koepfinger, M., Katsaras, J. and Kučerka, N.  2012. Molecular Structure of Fluid Phase Phosphatidylglycerol Bilayers as Determined by Small Angle Neutron and X-ray Scattering. Biochimica et Biophysica Acta 1818:2135-2148. (PDF) Supporting Information (PDF)


 13.  Hallinen, K.M., Tristram-Nagle, S. and Nagle, J.F. 2012.  Stability of Lipid  Bilayers.  Physical Chemistry Chemical Physics 14:15452-15457. (PDF)


 14.   Raghunathan, M., Zubovski, Y., Venable, R.M., Pastor, R.W., Nagle, J.F. and Tristram-Nagle, S. 2012. Structure and Elasticity of Lipid Membranes with Genistein and Daidzein Bioflavinoids using X-ray Scattering and MD Simulations. Journal of Physical Chemistry B 116:3918-3927. (PDF) Supporting Information (PDF) Cover (jpg)


 15.   Braun, A.R., Sevcsik, E., Chin, P., Rhoades, E., Tristram-Nagle, S. and Sachs, J.N. 2011. α-Synuclein Induces Both Positive Mean  Curvature and Negative Gaussian Curvature in Membranes. Journal of the American Chemical Society 134:1613-1620. (PDF)  Supporting Information (PDF)


 16.   Shchelokovskyy, P., Tristram-Nagle, S. and Dimova, R. 2011.  Effect of the HIV-1 Fusion Peptide on the Mechanical Properties and Leaflet Coupling of Lipid Bilayers. New Journal of Physics 13: 025004. (PDF)


 17.   Uppamoochikkal, P., Tristram-Nagle, S. and Nagle, J.F. 2010.  Orientation of Tie-lines in the Phase Diagram of DOPC/DPPC/ Cholesterol Model Biomembranes.  Langmuir 26: 17363–17368.  (PDF)


 18.   Tristram-Nagle, S., Chan, R., Kooijman, E.E., Uppamoochikkal, P., Qiang, W., Weliky, D.P. and Nagle, J.F. 2010.  HIV Fusion Peptide Penetrates, Disorders, and Softens T-cell Membrane Mimics. J. Mol. Biol. 402:139-153.  (PDF)


 19.   Tristram-Nagle, S., Kim, D., Akhunzada, N., Kučerka, N., Mathai, J.C., Katsaras, J., Zeidel, M. and Nagle, J.F. 2010.   Structure and Permeability of Fully Hydrated DiphytanoylPC.  Chem. Phys. Lipids 163:630-637.  (PDF)


 20.   Pan, J., Tristram-Nagle, S. and Nagle, J.F.  2009. Alamethicin Aggregation in Lipid Membranes.  J. Membrane Biol.. 231:11- 27.   (PDF)


 21.   Pan, J., Tristram-Nagle, S. and Nagle, J.F.  2009.  Effect of Cholesterol on Structural and Mechanical Properties of Membranes Depends on Lipid Chain Saturation.  Phys. Rev. E. 80:021931 (1-12).  (PDF)


 22.   Guler, S.D., Ghosh, D.D., Pan, J., Mathai, J.C., Zeidel, M.L., Nagle, J. F.  and Tristram-Nagle, S.  2009. Effects of Ether vs. Ester Linkage on Lipid Bilayer Structure and Water Permeability.   Chem. Phys. Lipids 160:33-44.  (PDF)


 23.   Pan, J., Tieleman, D. P., Nagle, J. F. , Kučerka, N., and Tristram-Nagle, S.  2009. Alamethicin in Lipid Bilayers: Combined Use  of X-Ray  Scattering and MD Simulations.  Biochim. Biophys. Acta 1788:1387-1397.  (PDF)


 24.   Kučerka, N., Perlmutter, J. D., Pan, J. J., Tristram-Nagle, S., Katsaras J., and Sachs, J. N. 2008. The Effect of Cholesterol on  Short- and Long-Chain Monounsaturated Lipid Bilayers as Determined by Molecular Dynamic Simulations and X-ray Scattering.   Biophys. J. 95:2792-2805.   (PDF).


 25.   Pan, J., Mills, T. T., Tristram-Nagle, S. and Nagle, J. F. 2008. Cholesterol Perturbs Lipid Bilayers Nonuniversally.  Phys. Rev.  Letts. 100:198103.   (PDF).


 26.   Mills, T. T., Toombes, G. E. S., Tristram-Nagle, S., Smilgies, D.-M., Feigenson, G. W. and Nagle, J. F. 2008. Order Parameters and Areas in Fluid-Phase Oriented Lipid Membranes Using Wide Angle X-ray Scattering. Biophys. J. 95, 669-681 (2008). (PDF)


 27.   Mills , T. T., Tristram-Nagle, S., Heberle, F. A., Morales, N. F., Zhao, J., Wu, J. W., Toombes, G. E. S., Nagle, J. F. and Feigenson, G. W. 2008. Liquid-Liquid Domains in Bilayers Detected by Wide Angle X-ray Scattering. Biophys. J. 95, 682-690   (2008). (PDF)


 28.   Greenwood , A. I., Pan , J., Mills, T. T., Nagle, J. F., Epand, R. M. and Tristram-Nagle, S. 2008. CRAC Motif Peptide of the HIV-1 gp41Protein Thins SOPC Membranes and Interacts with CholesterolBiochim. Biophys. Acta 1778:1120-1130.  (PDF).


 29.   Nagle, J. F., Mathai, J. C., Zeidel, M. L. and Tristram-Nagle, S. 2008. Theory of Passive Permeability through Lipid Bilayers. J.  Gen. Physiol. 131:77-85.  (PDF).


 30.   Mathai, J. C., Tristram-Nagle, S., Nagle, J. F. and Zeidel, M. L.  2008.  Structural Determinants of Water Permeability through the Lipid Membrane.  J. Gen. Physiol. 131:69-76. (PDF)


 31.    Pan, J., Tristram-Nagle, S. and Nagle, J.F. 2008. Temperature Dependence of Structure, Bending Rigidity and Bilayer  Interactions of DOPC  Bilayers. Biophys. J. 94:117-124. (PDF)


 32.   Tristram-Nagle, SPreparation of Oriented, Fully Hydrated Lipid Samples for Structure Determination Using X-Ray Scattering. 2007. Methods in Molecular Biology 400:(Methods in Membrane Lipids):63-75 (ed., A. Dopico), Humana Press, Totowa, NJ.    (PDF)


 33.   Tristram-Nagle, S. and Nagle, J.F. 2007.  HIV-1 Fusion Peptide Decreases Bending Energy, Promotes Curved Fusion    Intermediates. Biophysical Journal 93:2048-2055. (PDF


 34.      Kučerka, N., Tristram-Nagle, S. and Nagle, J.F. 2006. Closer Look at Structure of Fully Hydrated Fluid Phase DPPC Bilayers.  Biophysical Journal: Biophysical Letters 90:L83-85. (PDF)


 35.   Greenwood, A. I., Tristram-Nagle, S. and Nagle, J. F. 2006. Partial Molecular Volumes of Lipids and Cholesterol. Chem. Phys.  Lipids 143:1-10. (PDF)


 36.   Petrache, H.I., Tristram-Nagle, S., Harries, D., Kucerka, N., Nagle, J.F., and Parsegian, V.A. 2006. Swelling of Phospholipids by Monovalent Salt. J. Lipid Res. 47:302-309. (PDF)


 37.   Kučerka, N., Tristram-Nagle, S. and Nagle, J.F. 2005. Structure of Fully Hydrated Fluid Phase Lipid Bilayers with Monounsaturated Chains.  J.  Membrane Biol. 208: 193-202. (PDF)


 38.   Tristram-Nagle, S., Lewis, R.N.A.H., Blickenstaff, J. W., DiPrima, M., Marques, B. F., McElhaney, R. N., Nagle, J. F. and Schneider, J. W. 2005. Thermodynamic and Structural Characterization of Amino Acid-Linked Dialkyl Lipids.  Biophys. J.   86:1574-86.  (PDF).


 39.   Chu, N., Kučerka, N., Liu, Y., Tristram-Nagle, S., and Nagle, J. F. 2005. Anomalous Swelling of Lipid Bilayer Stacks Is Caused by Softening of the Bending Modulus.  Phys. Rev. E. 71:041904.  (PDF).


 40.   Kučerka, N., Liu, Y., Chu, N., Petrache, H.I., Tristram-Nagle, S., and Nagle, J. F. 2005. Structure of Fully Hydrated Fluid Phase DMPC and DLPC Bilayers Using X-Ray Scattering from Oriented Multilamellar Arrays and from Unilamellar Vesicles.    Biophys. J. 88:2626-2637.  (PDF).


 41.   Petrache, H.I., Tristram-Nagle, S., Gawrisch, K., Harries, D., Parsegian, V.A. and Nagle, J. F. 2004. Structure and Fluctuations of Charged Phosphatidylserine Bilayers In the Absence of SaltBiophys. J. 86:1574-86.  (PDF).


 42.   Tristram-Nagle, S. and Nagle, J. F. 2003. Lipid Bilayers: Thermodynamics, Structure, Fluctuations and InteractionsChem. Phys. Lipids (Invited Review) 127:3-14.  (PDF).


 43.   Eldho, N.V., Feller, S.E., Tristram-Nagle, S., Polozov, I.V. and Gawrisch, K. 2003. Polyunsaturated Docosahexaenoic vs. Docosapentaenoic Acid- Differences in Lipid Matrix Properties from the Loss of One Double Bond.  J. Am. Chem. Soc. 125:6409-6421.  (PDF).


 44.   Tristram-Nagle, S., Liu, Y., Legleiter, J. and Nagle, J. F. 2002. Structure of Gel Phase DMPC Determined by X-Ray Diffraction.  Biophys. J. 83:3324-3335.  (PDF).


 45.   Lyatskaya, Y., Liu, Y., Tristram-Nagle, S. , Katsaras, J. and Nagle, J.F. 2001. Method for Obtaining Structure and Interactions from Oriented Lipid Bilayers, Phys. Rev. E 63:011907 [1-9]. (PDF)  


 46.   Nagle, J.F. and Tristram-Nagle, S. 2000. Structure of Lipid Bilayers. (Invited Review for BBA). Biochim. Biophys. Acta  1469:159-195.  (PDF).


 47.   Nagle, J.F. and Tristram-Nagle, S. 2000. Lipid Bilayer Structure.  (Invited Review for Current Opinion in Structural Biology). COSB 10:474-480. (Postscript).


 48.   Katsaras, J., Tristram-Nagle, S., Liu, Y., Headrick, R.L., Fontes, E., Mason, P.C., Nagle, J.F. 2000. Clarification of the Ripple Phase of Lecithin Bilayers Using Fully Hydrated, Aligned Samples. Phys. Rev. E 61:5668-5677. (PDF)


 49.   Nagle, J.F. and Tristram-Nagle, S. 1999.  Structure and Interactions of Lipid Bilayers:  Role of Fluctuations.  In Lipid Bilayers,  eds. J. Katsaras,  T. Gutberlet, Springer Verlag, N.Y., (pp. 1-24.)  (Postscript).


 50.   Nagle, J.F., Liu, Y., Tristram-Nagle, S., Epand, R.M. and Stark, R. E.  1999.  Re-Analysis of Magic Angle Spinning NMR Determination of Intercellular WatersBiophys. J. 77:2062-2065. (PDF).


 51.   Tristram-Nagle, S., Isaacson, Y., Lyatskaya, Y., Liu, Y., Brummond, K., Katsaras, J. and Nagle, J.F.  1999.  Polymorphism in Myristoylpalmitoylphosphatidylcholine.  Chem. Phys. Lipids 100:101-113. (PDF)


 52.   Nagle, J.F., Petrache, H.I., Gouliaev, N., Tristram-Nagle, S., Liu, Y., Suter, R.M. and Gawrisch, K. 1998.  Multiple Mechanisms for Critical Behavior in the Biologically Relevant Phase of Lecithin Bilayers.  Phys. Rev. E. 58:7769-7776. (PDF)


Older papers are here

              Complete Publications are listed in a doc file.